对锌离子（Zn2+）的敏感性可区分典型肥大细胞和非典型肥大细胞。
The sensitivity to Zn2+ discriminates between typical and atypical mast cells.
作者信息
Mousli M, Gies J P, Bertrand C, Pelen F, Bronner C, Landry Y
机构信息
Laboratoire de Neuroimmunopharmacologie, Université Louis Pasteur-Strasbourg I, Faculté de Pharmacie, Illkirch, France.
出版信息
Agents Actions. 1990 Apr;30(1-2):102-5. doi: 10.1007/BF01969010.
The effects of zinc gluconate have been studied on rat peritoneal mast cells and rat basophilic leukemia cells (RBL 2H3) stimulated by various secretagogues. The IC50's of zinc gluconate on peritoneal cells were (microM): 1.6, 1.9, 5.4 and 18 for ionophore A23187, phorbol 12-myristate 13-acetate, substance P and immunoglobulin E-antigen, respectively. Higher concentrations of zinc gluconate were required to inhibit histamine secretion from RBL 2H3 cells, i.e. 12 microM (ionophore A23187) and 140 microM (immunoglobulin E-antigen). Zinc gluconate (10(-4) to 10(-3) M) also inhibited the IgE-dependent contraction of guinea pig trachea but was unable to affect that induced by exogenous histamine. These results suggest that zinc gluconate acts intracellularly and is selective of "typical" or "connective tissue" mast cells.
已研究葡萄糖酸锌对多种促分泌剂刺激的大鼠腹膜肥大细胞和大鼠嗜碱性白血病细胞（RBL 2H3）的作用。葡萄糖酸锌对腹膜细胞的半数抑制浓度（IC50，单位为微摩尔）：离子载体A23187为1.6、佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯为1.9、P物质为5.4、免疫球蛋白E - 抗原为18。抑制RBL 2H3细胞组胺分泌需要更高浓度的葡萄糖酸锌，即12微摩尔（离子载体A23187）和140微摩尔（免疫球蛋白E - 抗原）。葡萄糖酸锌（10⁻⁴至10⁻³ M）也抑制豚鼠气管的IgE依赖性收缩，但不能影响外源性组胺诱导的收缩。这些结果表明葡萄糖酸锌在细胞内起作用，并且对“典型”或“结缔组织”肥大细胞具有选择性。
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