Kitani S, Teshima R, Nonomura Y, Morita Y, Ito K
Department of Internal Medicine and Physical Therapy, University of Tokyo, Japan.
Int Arch Allergy Immunol. 1996 Aug;110(4):339-47. doi: 10.1159/000237326.
To study the involvement of serine/threonine phosphatase in the signal transduction of mast cells, we examined the effects of okadaic acid (OA), an inhibitor of type-1 and -2A phosphatase on histamine release, cell morphology, calcium influx and protein phosphorylation of rat basophilic leukemia (RBL-2H3) cells, human basophils and rat peritoneal mast cells. OA inhibited IgE-mediated histamine release from RBL-2H3 cells and human basophils dose-dependently. There was a remarkable enhancement of IgE-mediated histamine release when rat peritoneal mast cells were suboptimally challenged. OA induced a marked change of cell features, detached RBL-2H3 cells from plastic well and kept the 18- and 68-kD proteins phosphorylated. These findings show that phosphatase may play a role in the modulation of secretion in mast cells.
为研究丝氨酸/苏氨酸磷酸酶在肥大细胞信号转导中的作用，我们检测了冈田酸（OA），一种1型和2A型磷酸酶抑制剂，对大鼠嗜碱性白血病（RBL-2H3）细胞、人嗜碱性粒细胞和大鼠腹膜肥大细胞组胺释放、细胞形态、钙内流及蛋白质磷酸化的影响。OA剂量依赖性地抑制RBL-2H3细胞和人嗜碱性粒细胞中IgE介导的组胺释放。当对大鼠腹膜肥大细胞进行次优刺激时，IgE介导的组胺释放显著增强。OA诱导细胞特征发生明显变化，使RBL-2H3细胞从塑料孔中脱离，并使18-kD和68-kD蛋白保持磷酸化状态。这些发现表明，磷酸酶可能在肥大细胞分泌调节中发挥作用。
