与动物分离株具有相同间隔寡核苷酸分型图谱的多重耐药牛分枝杆菌分离株的分子流行病学
Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals.
作者信息
Romero Beatriz, Aranaz Alicia, de Juan Lucía, Alvarez Julio, Bezos Javier, Mateos Ana, Gómez-Mampaso Enrique, Domínguez Lucas
机构信息
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
J Clin Microbiol. 2006 Sep;44(9):3405-8. doi: 10.1128/JCM.00730-06.
Abstract
PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.
摘要
基于聚合酶链反应（PCR）的分型技术已被大多数实验室用于牛分枝杆菌分型。我们报告了对具有相同间隔寡核苷酸分型图谱的人源多重耐药牛分枝杆菌分离株和三株牛源分离株的分子特征分析。对直接重复区域的分析表明，两组在当前膜片中未包含的间隔序列存在情况上有所不同。在所测试的9个分枝杆菌散布重复单位可变数目串联重复序列位点中，有两个位点也能区分这两组，这表明人类感染并非源自分离株所来自的牛群。这些结果表明，即使对于那些出现频率较低的间隔寡核苷酸分型，也需要结合多种技术进行适当的鉴别。
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