Brecevic L, Michel S, Starke H, Müller K, Kosyakova N, Mrasek K, Weise A, Liehr T
School of Medicine, Croation Institute for Brain Research, Zagreb, Croatia.
Cytogenet Genome Res. 2006;114(3-4):319-24. doi: 10.1159/000094220.
There are only about 30 commercially available cell lines which include small supernumerary marker chromosomes (sSMC). As approximately 2.5 million people worldwide are carriers of an sSMC, this small number of immortalized cell lines is hard to understand. sSMC cell lines provide practically unlimited material for continuing studies e.g. to learn more about marker chromosome formation, or karyotypic evolution. To obtain information about their genetic content, in the present study we analyzed by FISH and multicolor-FISH approaches 19 sSMC cell lines obtained from the European Collection of Cell Cultures (ECACC). Microdissection and reverse painting, (sub-) centromere-specific multicolor-FISH (sub-)cenM-FISH, multicolor banding (MCB) and selected locus-specific FISH probes were applied. Thus, we were able to characterize comprehensively 14 out of 19 sSMC carrying cell lines; in the remaining five cases an sSMC could not be detected. Surprisingly, in six of the nine cell lines with sSMC previously characterized for their chromosomal origin by others, those results had to be revised. This has impact on the conclusions of previous studies, e.g. for uniparental disomy (UPD) in connection with sSMC.
仅有约30种可商购的细胞系包含小额外标记染色体（sSMC）。鉴于全球约有250万人是sSMC携带者，如此少量的永生化细胞系令人难以理解。sSMC细胞系为持续研究提供了几乎无限的材料，例如用于更多地了解标记染色体的形成或核型进化。为了获取有关其遗传内容的信息，在本研究中，我们通过荧光原位杂交（FISH）和多色FISH方法分析了从欧洲细胞培养物保藏中心（ECACC）获得的19个sSMC细胞系。应用了显微切割和反向染色体涂染、（亚）着丝粒特异性多色FISH（（亚）cenM-FISH）、多色显带（MCB）以及选定的位点特异性FISH探针。因此，我们能够全面表征19个携带sSMC的细胞系中的14个；在其余5个案例中未检测到sSMC。令人惊讶的是，在之前已被其他人根据染色体起源进行表征的9个带有sSMC的细胞系中，有6个的结果必须修正。这对先前研究的结论产生了影响，例如与sSMC相关的单亲二体性（UPD）研究结论。
