Indiani Chiara, O'Donnell Mike
Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10021, USA.
Nat Rev Mol Cell Biol. 2006 Oct;7(10):751-61. doi: 10.1038/nrm2022. Epub 2006 Sep 6.
Sliding clamps are ring-shaped proteins that tether DNA polymerases to DNA, which enables the rapid and processive synthesis of both leading and lagging strands at the replication fork. The clamp-loading machinery must repeatedly load sliding-clamp factors onto primed sites at the replication fork. Recent structural and biochemical analyses provide unique insights into how these clamp-loading ATPase machines function to load clamps onto the DNA. Moreover, these studies highlight the evolutionary conservation of the clamp-loading process in the three domains of life.
滑动夹是环形蛋白质，可将DNA聚合酶与DNA相连，从而在复制叉处实现前导链和后随链的快速且持续合成。夹加载机制必须反复将滑动夹因子加载到复制叉处的引发位点上。最近的结构和生化分析为这些夹加载ATP酶机器如何将夹子加载到DNA上发挥作用提供了独特见解。此外，这些研究突出了夹加载过程在生命三个域中的进化保守性。
