疟蚊斯氏按蚊中新的假定c型溶菌酶的鉴定与特性分析
Identification and characterization of a new putative c-type lysozyme from malaria vector Anopheles stephensi.
作者信息
Dixit Rajnikant, Dixit Sarita, Gakhar Surendra
机构信息
Department of Biosciences, Maharashi Dayanand University, Rohtak, India.
出版信息
Indian J Biochem Biophys. 2006 Feb;43(1):15-9.
Lysozyme (E.C. 3.2.1.17) activity is reported from the malaria vector Anopheles stephensi. The activity was detected in the salivary gland and midgut using bacteriolytic radial diffusion assay. Spectrophotometric analysis indicated that higher level of lysozyme activity was maintained in both midgut and salivary gland tissues. The activity reached the highest level in 4-8 days old mosquitoes. Genomic PCR amplification revealed the presence of at least two putative lysozyme genes in the mosquito genome. Preliminary analysis of one of the 413 bp genomic fragments showed 56% identity to the lysozyme of mosquito A. gambiae. However, the nature and origin of the putative cloned lysozyme gene remains elusive.
据报道，疟蚊斯氏按蚊具有溶菌酶（E.C. 3.2.1.17）活性。使用溶菌径向扩散测定法在唾液腺和中肠中检测到了该活性。分光光度分析表明，中肠和唾液腺组织中均维持着较高水平的溶菌酶活性。在4至8日龄的蚊子中，该活性达到最高水平。基因组PCR扩增显示，蚊子基因组中存在至少两个推定的溶菌酶基因。对413 bp基因组片段之一的初步分析表明，其与冈比亚按蚊的溶菌酶有56%的同一性。然而，推定克隆的溶菌酶基因的性质和来源仍然不明。
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