Rosenfeld A, Vanderberg J P
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10016, USA.
J Parasitol. 1998 Apr;84(2):361-5.
Digestion of blood within the mosquito midgut is mediated primarily by a series of proteases, and several previous studies have described protease activity within homogenates of the midgut of the malaria vector Anopheles stephensi. We have expanded on these previous data by resolving protease isoforms from the midgut as well as the hemolymph of adult An. stephensi mosquitoes via gel electrophoresis and zymography. Using this procedure, we have been able to identify multiple isozymes of trypsin, chymotrypsin, and aminopeptidase. We were able to detect an increase in the intensity of some of these protease bands plus the appearance of new bands 24 hr after mosquitoes had taken a blood meal. Furthermore, we detected 2 endogenous trypsin isozymes within the hemolymph. There was no upregulation of these hemolymph isozymes after a blood meal, thus suggesting that they may not be involved in digestion of the blood meal by the mosquito.
蚊子中肠内血液的消化主要由一系列蛋白酶介导，之前的几项研究已经描述了疟蚊媒介斯氏按蚊中肠匀浆中的蛋白酶活性。我们通过凝胶电泳和酶谱法解析成年斯氏按蚊中肠以及血淋巴中的蛋白酶同工型，对这些先前的数据进行了扩展。使用这个程序，我们能够鉴定出胰蛋白酶、胰凝乳蛋白酶和氨肽酶的多种同工酶。我们能够检测到蚊子吸食血餐24小时后，其中一些蛋白酶条带的强度增加以及新条带的出现。此外，我们在血淋巴中检测到2种内源性胰蛋白酶同工酶。血餐之后这些血淋巴同工酶没有上调，因此表明它们可能不参与蚊子对血餐的消化。
