Wang Quanzhen, Wang Fabin, Hong Guangxiang
Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, 430022, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Aug;20(8):805-8.
To study the effect of the competitive inhibitor of nitric oxide synthase N(G)-nitro-L-arginine methyl ester (L-NAME) on the denervated muscle atrophy.
A model of the denervated gastrocnemius at the right lower limb was established in 36 SD adult rats. The rats were randomly divided into two groups: the L-NAME group (Group A) and the control group (Group B). L-NAME 10 mg/kg daily was injected into the denervated gastrocnemius in Group A, and normal saline was injected into the denervated gastrocnemius in Group B. At 2, 4 and 8 weeks after operation, the rate of the muscle wet weight preservation, the cross section area of the myocyte, the protein amount, and the percentage of the apoptotic muscle cells were measured respectively and the ultramicrostructure of the myocyte was observed.
At 2 and 4 weeks after operation, the rate of the muscle wet weight preservation, the cross section area of the myocyte, and the protein amount were significantly greater in Group A than in Group B; however, the percentage of the apoptotic muscle cells was significantly smaller in Group A than in Group B. The observation of the ultramicrostructure of the myocyte showed that an injection of L-NAME could protect the ultramicrostructure of the myocyte. At 8 weeks after operation, there was no significant difference between the two groups in the above-mentioned parameters.
The nitric oxide synthase inhibition can delay the denervated muscle atrophy.
研究一氧化氮合酶竞争性抑制剂N（G）-硝基-L-精氨酸甲酯（L-NAME）对失神经支配肌肉萎缩的影响。
将36只成年SD大鼠建立右下肢失神经支配的腓肠肌模型。大鼠随机分为两组：L-NAME组（A组）和对照组（B组）。A组对失神经支配的腓肠肌每日注射L-NAME 10 mg/kg，B组对失神经支配的腓肠肌注射生理盐水。术后2、4和8周，分别测量肌肉湿重保留率、肌细胞横截面积、蛋白含量以及凋亡肌细胞百分比，并观察肌细胞超微结构。
术后2周和4周，A组肌肉湿重保留率、肌细胞横截面积和蛋白含量均显著高于B组；然而，A组凋亡肌细胞百分比显著低于B组。肌细胞超微结构观察显示，注射L-NAME可保护肌细胞超微结构。术后8周，两组上述参数无显著差异。
一氧化氮合酶抑制可延缓失神经支配肌肉萎缩。
