Schulz R A, Xie X L, Miksch J L
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Dev Biol. 1990 Aug;140(2):455-8. doi: 10.1016/0012-1606(90)90094-y.
We have identified two DNA sequences required for the proper germ line expression of the Drosophila gonadal (gdl) gene. A 53-bp region located from -328 to -276 relative to the gdlM transcription start site was required for the expression of a gdl-lacZ gene fusion in the male germ line. This sequence resides within the upstream z600 gene; however, z600 expression was undetectable in male germ cells. A 30-bp region located from -5 to +25 relative to the gdlF transcription start site was required for the expression of gdl-lacZ gene fusion in the female germ line.
我们已经鉴定出果蝇性腺（gdl）基因在生殖系中正常表达所需的两个DNA序列。相对于gdlM转录起始位点，位于-328至-276的一个53bp区域是gdl-lacZ基因融合体在雄性生殖系中表达所必需的。该序列位于上游z600基因内；然而，在雄性生殖细胞中未检测到z600的表达。相对于gdlF转录起始位点，位于-5至+25的一个30bp区域是gdl-lacZ基因融合体在雌性生殖系中表达所必需的。
