Sassenscheidt Julian, Rohayem Jacques, Illmer Thomas, Bandt Dirk
Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Medizinisch-Theoretisches Zentrum, Fiedlerstrasse 42, 01307 Dresden, Germany.
J Virol Methods. 2006 Dec;138(1-2):40-8. doi: 10.1016/j.jviromet.2006.07.015. Epub 2006 Sep 7.
The aim of this study was to investigate the clinical impact of reactivation of human herpes virus-6 (HHV-6) and HHV-7 infections in stem cell transplantation recipients, and to examine a possible increase in virulence of the two roseoloviruses when a reactivation of CMV (HHV-5) simultaneously occurs. For this purpose, quantitative real-time PCR systems were developed to assess the viral load of CMV, HHV-6, or HHV-7 in the plasma of haematopoetic stem cell recipients. One hundred and ninety-eight plasma samples from 37 patients who underwent allogeneic stem cell transplantation were tested for CMV, HHV-6, and HHV-7 by a 5'-exonuclease (TaqMan) quantitative real-time PCR. The CMV load obtained by the real-time PCR assay was compared retrospectively with results generated previously with a commercially available test (COBAS AMPLICOR CMV MONITOR Test, Roche). The results suggest that CMV and HHV-6 may be associated with post-transplantation end-organ disease, while HHV-7 reactivation had no impact on the patients included in this study. No evidence for a potential interaction of the roseoloviruses and CMV infections was found.
本研究的目的是调查人类疱疹病毒6型（HHV-6）和HHV-7感染再激活对干细胞移植受者的临床影响，并研究当巨细胞病毒（HHV-5）同时再激活时这两种玫瑰疹病毒的毒力是否可能增加。为此，开发了定量实时PCR系统，以评估造血干细胞移植受者血浆中CMV、HHV-6或HHV-7的病毒载量。通过5'-核酸外切酶（TaqMan）定量实时PCR对37例接受异基因干细胞移植患者的198份血浆样本进行CMV、HHV-6和HHV-7检测。将实时PCR检测获得的CMV载量与先前使用市售检测方法（COBAS AMPLICOR CMV MONITOR Test，罗氏公司）所得结果进行回顾性比较。结果表明，CMV和HHV-6可能与移植后终末器官疾病有关，而HHV-7再激活对本研究中的患者没有影响。未发现玫瑰疹病毒与CMV感染之间存在潜在相互作用的证据。
