Halverson M A, Skelly D K, Caccone A
School of Forestry and Environmental Studies, Yale University, 370 Prospect Street, New Haven, CT 06520, USA.
J Hered. 2006 Sep-Oct;97(5):499-507. doi: 10.1093/jhered/esl019. Epub 2006 Sep 6.
We examined the effects of inbreeding on the performance of wood frog (Rana sylvatica) larvae in the field and in the laboratory. We used microsatellite analysis to establish the parentage and degree of inbreeding of the larvae. Two different estimators of inbreeding were used. The first was based on average multilocus heterozygosity, and the second was based on a molecular relatedness estimator. The estimators were highly correlated, and both showed a significant negative relationship between inbreeding and survival in the wild. However, there was no evidence that inbreeding influenced growth or development in the wild. Neither was there any evidence that inbreeding affected survival, growth, or development in the laboratory. These results suggest that, for wood frogs, inbreeding has a bigger effect on fitness in the wild than in captivity and that measurements of survival are more sensitive than measures of growth or development.
我们研究了近亲繁殖对林蛙（Rana sylvatica）幼虫在野外和实验室环境下表现的影响。我们使用微卫星分析来确定幼虫的亲本关系和近亲繁殖程度。使用了两种不同的近亲繁殖估计方法。第一种基于平均多位点杂合性，第二种基于分子亲缘关系估计器。这两种估计器高度相关，并且都显示出近亲繁殖与野外生存率之间存在显著的负相关关系。然而，没有证据表明近亲繁殖会影响野外的生长或发育。也没有任何证据表明近亲繁殖会影响实验室中的生存、生长或发育。这些结果表明，对于林蛙来说，近亲繁殖对野外适应性的影响比对圈养环境的影响更大，并且生存测量比生长或发育测量更敏感。
