Kosta Artemis, Laporte Catherine, Lam David, Tresse Emilie, Luciani Marie-Françoise, Golstein Pierre
Centre d'Immunologie INSERM-CNRS-Univ. Medit. de Marseille-Luminy, France.
Methods Mol Biol. 2006;346:535-50. doi: 10.1385/1-59745-144-4:535.
In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using minor modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells or the corresponding atg1- autophagy gene mutant cells. Methods to follow cell death qualitatively and quantitatively facilitate detailed studies of vacuolar death in wild-type cells and of nonvacuolar, "condensed" death in atg1- mutant cells.
在本章中，我们描述了如何通过简单的细胞培养、克隆形成试验以及光子显微镜（借助染色技术）和电子显微镜，方便地在盘基网柄菌中展示、评估和研究细胞死亡。使用对Rob Kay等人的单层技术进行微小修改的方法，以及野生型HMX44A盘基网柄菌细胞或相应的atg1自噬基因缺陷型细胞，都可以方便地诱导细胞死亡。定性和定量追踪细胞死亡的方法有助于对野生型细胞中的液泡死亡以及atg1缺陷型细胞中的非液泡“浓缩”死亡进行详细研究。
