Streicher Werner W, Makhatadze George I
Department of Biochemistry and Molecular Biology, Penn State College of Medicine, Hershey, PA, USA.
Methods Mol Biol. 2007;350:105-13. doi: 10.1385/1-59745-189-4:105.
Differential scanning calorimetry can measure the heat capacity of a protein/peptide solution over a range of temperatures at constant pressure, which is used to determine the enthalpy function of the system. There are several experimental factors that can have a significant impact on the determined enthalpy and subsequent derived thermodynamic parameters. These factors are discussed in terms of sample and instrument preparation, as well as data collection and analysis.
