Pagesy P, Li J Y, Rentier-Delrue F, Delalande O, Le Bouc Y, Kujas M, Joubert D, Martial J, Peillon F
INSERM U. 223, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Acta Endocrinol (Copenh). 1990 Jun;122(6):745-52. doi: 10.1530/acta.0.1220745.
Some patients with active acromegaly have elevated plasma IGF-I concentrations with only minimal elevation of plasma GH. We compared adenomatous GH and SRIH expression in 3 such patients (patients No. 1, 2 and 3; basal plasma GH level less than 4 micrograms/l) and in 3 acromegalic patients with high basal plasma GH level (patients No. 4, 5 and 6; 51.7 +/- 16.1 micrograms/l, mean +/- SEM). By immunocytochemistry, all the tumours proved to be somatotropic adenomas. At the ultrastructural level, signs of low secretory activity were observed in adenomas from patients No. 2 and 3. Perifused adenoma cells of patients No. 1, 2 and 3 released very little GH compared with those of patients No. 4, 5 and 6 (1 +/- 0.37 vs 51.5 +/- 34.1 micrograms x (10(-6) cells) x min-1, p less than 0.001). Adenoma SRIH content was 65.7 and 30.6 pg/mg proteins in patients No. 1 and 2, whereas it was undetectable in the others (patients No. 4, 5 and 6). Northern blot analysis showed that the GH gene was poorly expressed in the adenomas from patients No. 1, 2 and 3 compared with the adenomas from patients No. 4, 5 and 6. SRIH mRNA was detected in all 6 adenomas. However, the signal was more intense in the adenomas from patients No. 1, 2 and 3 than in those from patients No. 4, 5 and 6.(ABSTRACT TRUNCATED AT 250 WORDS)
一些患有活动性肢端肥大症的患者血浆胰岛素样生长因子-I（IGF-I）浓度升高，而血浆生长激素（GH）仅有轻微升高。我们比较了3例此类患者（1号、2号和3号患者；基础血浆GH水平低于4微克/升）和3例基础血浆GH水平高的肢端肥大症患者（4号、5号和6号患者；51.7±16.1微克/升，平均值±标准误）腺瘤性GH和生长抑素（SRIH）的表达。通过免疫细胞化学分析，所有肿瘤均为生长激素细胞腺瘤。在超微结构水平上，2号和3号患者的腺瘤中观察到分泌活性低的迹象。与4号、5号和6号患者相比，1号、2号和3号患者的灌流腺瘤细胞释放的GH非常少（1±0.37微克/（10⁻⁶个细胞）×分钟⁻¹ 对51.5±34.1微克/（10⁻⁶个细胞）×分钟⁻¹，p<0.001）。1号和2号患者腺瘤的SRIH含量分别为65.7和30.6皮克/毫克蛋白质，而其他患者（4号、5号和6号患者）未检测到。Northern印迹分析显示，与4号、5号和6号患者的腺瘤相比，1号、2号和3号患者的腺瘤中GH基因表达较差。所有6例腺瘤中均检测到SRIH mRNA。然而，1号、2号和3号患者腺瘤中的信号比4号、5号和6号患者腺瘤中的信号更强。（摘要截短于250字）
