Papan Cyrus, Boulat Benoit, Velan S Sendhil, Fraser Scott E, Jacobs Russell E
Beckman Institute 139-74, California Institute of Technology, Pasadena, California, USA.
Dev Dyn. 2006 Nov;235(11):3059-62. doi: 10.1002/dvdy.20947.
Mitotic cell division is a highly regulated cellular event in all organisms, but its direct visualization in the vertebrates is limited to animals with transparent embryos. Here, we report on the use of microscopic magnetic resonance imaging (mMRI) to noninvasively observe mitotic cell division of early blastomeres in the optically opaque Xenopus laevis embryo. Due to intrinsic tissue contrast, cell nuclei can be directly visualized without the need for contrast enhancing labeling. By taking two-dimensional in vivo time-lapse image sequences, the karyokinesis of a blastomere is followed. Timing and orientation of the cleavages can be traced for five cell divisions to establish a cell lineage tree, including orientation and timing of the mitosis. This work demonstrates for the first time the use of MRI for the visualization of cell divisions and expands the experimental scope of the Xenopus embryo.
有丝分裂细胞分裂是所有生物体中高度受调控的细胞事件，但其在脊椎动物中的直接可视化仅限于具有透明胚胎的动物。在此，我们报告了使用显微磁共振成像（mMRI）对不透明的非洲爪蟾胚胎早期卵裂球的有丝分裂细胞分裂进行无创观察。由于内在的组织对比度，无需进行增强对比度的标记即可直接观察到细胞核。通过获取二维体内延时图像序列，追踪一个卵裂球的核分裂。可以追踪五次细胞分裂的分裂时间和方向，以建立细胞谱系树，包括有丝分裂的方向和时间。这项工作首次证明了使用MRI可视化细胞分裂，并扩展了非洲爪蟾胚胎的实验范围。
