Eales Kathryn L, Nielsen Jeppe L, Seviour Elizabeth M, Nielsen Per H, Seviour Robert J
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3550, Australia.
Environ Microbiol. 2006 Oct;8(10):1712-20. doi: 10.1111/j.1462-2920.2006.01107.x.
The in situ physiology of the filamentous bacterium Skermania piniformis frequently seen in activated sludge foams in Australia was investigated. An oligonucleotide probe, Spin1449, targeting the 16S rRNA of S. piniformis was designed for its identification by fluorescence in situ hybridization (FISH), validated with pure cultures and applied successfully to foam samples from two geographically distant Australian plants. While filaments of this bacterium appeared to be comparatively hydrophobic, the organism had no clear preference for hydrophobic or hydrophilic substrates. In both foams examined using microautoradiography (MAR), filaments selectively took up substrates under aerobic and anoxic (NO(3) (-)) but not anaerobic or anoxic (NO(2) (-)) conditions. Skermania piniformis assimilated oleic acid, palmitic acid, glycerol and glycine. Ectoenzyme activities detected suggest that S. piniformis has an ability to assimilate a greater range of substrates than might be concluded from the MAR data obtained here. Based on the substrate uptake data presented here, an anaerobic selector may work for controlling S. piniformis in activated sludge systems.
对澳大利亚活性污泥泡沫中常见的丝状细菌斯氏丝状菌（Skermania piniformis）的原位生理学进行了研究。设计了一种靶向斯氏丝状菌16S rRNA的寡核苷酸探针Spin1449，用于通过荧光原位杂交（FISH）对其进行鉴定，该探针经纯培养物验证，并成功应用于来自澳大利亚两个地理位置遥远的工厂的泡沫样品。虽然这种细菌的丝状体似乎具有相对较强的疏水性，但该生物体对疏水或亲水底物没有明显偏好。在使用微放射自显影（MAR）检查的两种泡沫中，丝状体在有氧和缺氧（NO₃⁻）条件下选择性摄取底物，但在厌氧或缺氧（NO₂⁻）条件下则不然。斯氏丝状菌同化油酸、棕榈酸、甘油和甘氨酸。检测到的胞外酶活性表明，斯氏丝状菌同化底物的范围可能比此处获得的MAR数据所显示的更广。基于此处呈现的底物摄取数据，厌氧选择器可能有助于控制活性污泥系统中的斯氏丝状菌。
