分离的叶绿体分裂机制在伸展后能够主动收缩。

Isolated chloroplast division machinery can actively constrict after stretching.

作者信息

Yoshida Yamato, Kuroiwa Haruko, Misumi Osami, Nishida Keiji, Yagisawa Fumi, Fujiwara Takayuki, Nanamiya Hideaki, Kawamura Fujio, Kuroiwa Tsuneyoshi

机构信息

Laboratory of Cell Biology, Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima, Tokyo 171-8501, Japan.

出版信息

Science. 2006 Sep 8;313(5792):1435-8. doi: 10.1126/science.1129689.

DOI:10.1126/science.1129689

PMID:16960006

Abstract

Chloroplast division involves plastid-dividing, dynamin, and FtsZ (PDF) rings. We isolated intact supertwisted (or spiral) and circular PDF machineries from chloroplasts of the red alga Cyanidioschyzon merolae. After individual intact PDF machineries were stretched to four times their original lengths with optical tweezers, they spontaneously returned to their original sizes. Dynamin-released PDF machineries did not retain the spiral structure and could not be stretched. Thus, dynamin may generate the motive force for contraction by filament sliding in dividing chloroplasts, in addition to pinching-off the membranes.

摘要

叶绿体分裂涉及质体分裂、发动蛋白和FtsZ（PDF）环。我们从红藻梅氏嗜热栖热菌的叶绿体中分离出完整的超螺旋（或螺旋状）和环状PDF机器。在用光镊将单个完整的PDF机器拉伸至其原始长度的四倍后，它们会自发恢复到原始大小。发动蛋白释放后的PDF机器无法保持螺旋结构，也无法被拉伸。因此，发动蛋白除了在叶绿体分裂时夹断膜之外，还可能通过细丝滑动产生收缩的动力。