Hannon J A, Kilcawley K N, Wilkinson M G, Delahunty C M, Beresford T P
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
J Dairy Sci. 2006 Oct;89(10):3749-62. doi: 10.3168/jds.S0022-0302(06)72416-X.
Fast-ripened Cheddar cheeses for ingredient purposes were produced by addition of a dried enzyme-modified cheese (EMC; 0.25 and 1 g/100 g of milled curd) at the salting stage during a standard Cheddar cheese-making procedure. Populations of starter and nonstarter lactic acid bacteria (NSLAB), levels of proteolysis and lipolysis, volatile analysis, and flavor development (by quantitative descriptive sensory analysis) were monitored over a 6-mo ripening period. Levels of free AA and free fatty acids were elevated in the experimental cheeses on d 1 because of inclusion of the EMC. Counts of NSLAB were also elevated in the experimental cheeses compared with the control cheese from the start of ripening. Levels of free AA were slightly elevated in the experimental cheeses at 1, 2, and 4 mo, but significantly greater accumulations were detected by 6 mo of ripening, with His, Leu, and glutamate reflecting the greatest increases. Levels of long-chain free fatty acids increased up to 2 mo, indicating an initial stimulation of lipolysis, but had decreased by 6 mo, indicating greater catabolism, probably caused by NSLAB and increased starter lysis. Principal component analysis of the volatile compounds showed few differences in the aroma profiles among the cheeses up to 4 mo of ripening, but a large separation of the cheeses supplemented with EMC relative to the control was observed by 6 mo. Sensory analysis of the cheeses with added EMC showed an acceleration of 2 mo in flavor development compared with the control cheese with the addition of 1 g/100 g of EMC developing a flavor profile at 4 mo similar to the control cheese at 6 mo of ripening. However, atypical Cheddar flavors developed on prolonged storage. This study shows the potential of adding EMC during Cheddar production to produce a fast-ripened ingredient-type Cheddar cheese.
在标准切达干酪制作流程的腌制阶段,通过添加干酶改性干酪(EMC;0.25克和1克/100克磨碎凝乳)来生产用于配料的快速成熟切达干酪。在6个月的成熟期间,监测了发酵剂和非发酵剂乳酸菌(NSLAB)的数量、蛋白水解和脂肪水解水平、挥发性成分分析以及风味发展(通过定量描述性感官分析)。由于添加了EMC,实验干酪在第1天的游离氨基酸和游离脂肪酸水平升高。与对照干酪相比,从成熟开始,实验干酪中NSLAB的数量也有所增加。实验干酪在1、2和4个月时游离氨基酸水平略有升高,但在成熟6个月时检测到显著更多的积累,其中组氨酸、亮氨酸和谷氨酸的增加最为明显。长链游离脂肪酸水平在2个月内升高,表明脂肪水解最初受到刺激,但在6个月时有所下降,表明分解代谢增强,这可能是由NSLAB和发酵剂裂解增加所致。挥发性化合物的主成分分析表明,在成熟4个月之前,各干酪的香气特征差异不大,但在6个月时,添加EMC的干酪与对照干酪相比有很大分离。添加EMC的干酪的感官分析表明,与对照干酪相比,风味发展加速了2个月,添加1克/100克EMC的干酪在4个月时形成的风味特征与对照干酪在成熟6个月时相似。然而,长时间储存会产生非典型切达风味。本研究表明,在切达干酪生产过程中添加EMC有潜力生产出快速成熟的配料型切达干酪。