Evaluation of AS185 as an adjunct culture in probiotic cheddar cheese manufacture.

A novel probiotic AS185, isolated from traditional Chinese fermented foods, was used as an adjunct culture for probiotic cheddar cheese production. The physicochemical composition, textural, free amino acids (FAAs), short-chain fatty acids (SCFAs) profiles, sensory properties, and microbial survival, was evaluated during the 90-day ripening period. The addition of AS185 did not influence the physicochemical composition of cheddar cheese but significantly decreased the hardness without affecting its textural profile. During ripening, AS185 was able to grow and promote the generation of FAAs and SCFAs, but did not alter the overall sensory properties; it rather improved the flavor and taste of cheese. In addition, the cheese matrix protected strain AS185 during transit throughout the simulated gastrointestinal system. These results demonstrated that AS185 adjunct cultures might be useful for producing high-quality probiotic cheddar cheese.