Lahdenne P, Heikinheimo M, Perkkiö M, Rapola J, Miettinen M
Children's Hospital, University of Helsinki, Finland.
Pathol Res Pract. 1990 Jun;186(3):336-43. doi: 10.1016/S0344-0338(11)80292-X.
The cell differentiation properties of thirty-four sacrococcygeal teratomas (SCT) and their five recurrences were immunohistochemically studied for the expression of different classes of intermediate filament proteins, muscle actin (MA) and S-100 protein. Out of thirty-nine tumors twenty-three were SCTs with only mature tissue elements, seven immature teratomas, five pure endodermal sinus tumors (EST) and four ESTs or embryonal carcinomas (EC) combined with mature components. Cytokeratin positivity was found in all epithelial structures and sometimes also in smooth muscle and primitive mesenchymal cells. An intense cytokeratin immunoreactivity was observed in EC and EST components. Muscle markers, desmin and MA were present in smooth and striated muscle cells. Focal desmin positivity was also found in some epithelial structures in two cases. Glial tissue positive for glial fibrillary acidic protein (GFAP) was found in twenty-eight out of thirty-nine tumors. Some cases with no apparent glial tissue in hematoxylin and eosin staining showed glial differentiation as proved by GFAP positivity. In six out of eleven choroid plexus-like tissues GFAP positive cells were observed. S-100 protein showed an intense distribution of immunoreactivity outside neural tissue, and focal positivity was observed in malignant epithelial structures. Immunohistochemical markers did not reveal any prognostic significance in teratomas. Our findings, however, showed some aberrant features of cell differentiation from normal mature tissue components but closely parallel to those found in normal fetal development.
对34例骶尾部畸胎瘤（SCT）及其5例复发肿瘤的细胞分化特性进行了免疫组织化学研究，以检测不同类型中间丝蛋白、肌动蛋白（MA）和S-100蛋白的表达。在39个肿瘤中，23个为仅含有成熟组织成分的SCT，7个为未成熟畸胎瘤，5个为纯内胚窦瘤（EST），4个为合并成熟成分的EST或胚胎癌（EC）。在所有上皮结构中均发现细胞角蛋白阳性，有时在平滑肌和原始间充质细胞中也呈阳性。在EC和EST成分中观察到强烈的细胞角蛋白免疫反应性。肌肉标志物结蛋白和MA存在于平滑肌和横纹肌细胞中。在2例中，在一些上皮结构中也发现了局灶性结蛋白阳性。在39个肿瘤中的28个中发现了胶质纤维酸性蛋白（GFAP）阳性的神经胶质组织。一些在苏木精和伊红染色中无明显神经胶质组织的病例，经GFAP阳性证实存在神经胶质分化。在11个脉络丛样组织中的6个中观察到GFAP阳性细胞。S-100蛋白在神经组织外显示出强烈分布的免疫反应性，在恶性上皮结构中观察到局灶性阳性。免疫组织化学标志物在畸胎瘤中未显示出任何预后意义。然而，我们的研究结果显示，细胞分化与正常成熟组织成分存在一些异常特征，但与正常胎儿发育中的情况密切平行。
