Takeda Y, Takeda J, Smart B M, Krause J E
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Regul Pept. 1990 May 21;28(3):323-33. doi: 10.1016/0167-0115(90)90030-z.
Substance P (SP) and multiple neurokinin A (NKA)-related peptides can be derived from alpha-, beta- and/or gamma-preprotachykinin (PPT) mRNAs. In this study, the relative concentrations of the tachykinin peptides derived from the SP gene in rat brain, duodenum, jejunum, submandibular gland, parotid gland, urinary bladder and vas deferens was determined using high-performance liquid chromatography (HPLC) and radioimmunoassays (RIAs). In all tissues, SP levels were the highest. The relative abundance of NKA-related peptides was NKA greater than neuropeptide gamma (NP gamma) = neuropeptide K (NPK) greater than NKA(3-10). These results demonstrate that multiple tachykinin peptides are present in tissues where the SP gene is expressed, and that the NKA portion of the beta- and gamma-PPT precursors can be differentially processed posttranslationally in rat tissues into NKA, NPK, NP gamma and/or NKA(3-10).
P物质（SP）和多种神经激肽A（NKA）相关肽可源自α-、β-和/或γ-前速激肽原（PPT）mRNA。在本研究中，使用高效液相色谱法（HPLC）和放射免疫分析法（RIA）测定了大鼠脑、十二指肠、空肠、下颌下腺、腮腺、膀胱和输精管中源自SP基因的速激肽肽的相对浓度。在所有组织中，SP水平最高。NKA相关肽的相对丰度为NKA大于神经肽γ（NPγ）=神经肽K（NPK）大于NKA（3-10）。这些结果表明，在表达SP基因的组织中存在多种速激肽肽，并且β-和γ-PPT前体的NKA部分在大鼠组织中可在翻译后被差异加工成NKA、NPK、NPγ和/或NKA（3-10）。
