Citron M, Schuster H
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Cell. 1990 Aug 10;62(3):591-8. doi: 10.1016/0092-8674(90)90023-8.
The c4 repressors of P1 and P7 inhibit antirepressor synthesis and are solely responsible for heteroimmunity of the phages. We show that c4 is a new type of antisense RNA acting on a target, ant mRNA, that is transcribed from the same promoter. Interaction depends on complementarity of two pairs of short sequences encompassing the ribosome binding site involved in ant expression. We demonstrate that heteroimmunity of P1 and P7 is due to just two substitutions in each of the complementary sequences of c4 and ant mRNA. Based on P1-P7 sequence comparison and a mutant analysis, we propose a secondary structure model for c4 RNA, with the complementary regions in loops as important sites for antisense control.
P1和P7的c4阻遏物抑制抗阻遏物的合成，并且是噬菌体异种免疫的唯一原因。我们发现c4是一种新型的反义RNA，作用于从同一启动子转录而来的靶标——ant mRNA。相互作用取决于两对短序列的互补性，这两对短序列包含了参与ant表达的核糖体结合位点。我们证明P1和P7的异种免疫仅仅是由于c4和ant mRNA的互补序列中各自发生了两个取代。基于P1 - P7序列比较和突变分析，我们提出了c4 RNA的二级结构模型，其中环中的互补区域是反义控制的重要位点。
