Leroy E, Madariaga L, Ben Aribia M, Mishal Z, Theodorou I, Rochant H, Vernant J P, Senik A
Laboratoire d'Immunologie Cellulaire et de Transplantation, I.R.S.C., Villejuif, France.
Exp Hematol. 1990 Aug;18(7):770-4.
We analyzed the functional status of the small CD8+/Leu-7+ T-lymphocytes that circulate in increased proportions in the blood of many allogeneic bone marrow transplant (BMT) patients. Purified CD8+/Leu-7+ T cells were tested for their effect on T-cell proliferative responses. In contrast to CD8+/Leu-7-T-lymphocytes, such cells behaved as suppressor cells for lectin-induced mitogenic responses of the donor's peripheral blood lymphocytes. However, they did not interfere with the in vitro responsiveness to specific stimuli such as protein purified derivative (PPD) or alloantigens. We demonstrate that CD8+/Leu-7+ T cells are resting pre-cytotoxic T-lymphocytes (CTL) that can be induced by mitogenic lectins to express their cytolytic program in a non-specific, non-major histocompatibility complex-restricted manner against phytohemagglutinin-treated lymphoblasts or K562 target cells. The lectin-triggered cytotoxicity was achieved within a few days, together with limited cell division. Our results suggest that circulating CD8+/Leu-7+ T cells from BMT recipients are in vivo primed CTL awaiting cellular activation.
我们分析了许多异基因骨髓移植（BMT）患者血液中循环比例增加的小CD8⁺/Leu-7⁺T淋巴细胞的功能状态。对纯化的CD8⁺/Leu-7⁺T细胞对T细胞增殖反应的影响进行了检测。与CD8⁺/Leu-7⁻T淋巴细胞相反，这类细胞对供体外周血淋巴细胞的凝集素诱导的有丝分裂反应起抑制细胞的作用。然而，它们并不干扰对特定刺激（如蛋白纯化衍生物（PPD）或同种异体抗原）的体外反应性。我们证明，CD8⁺/Leu-7⁺T细胞是静止的前细胞毒性T淋巴细胞（CTL），有丝分裂凝集素可诱导它们以非特异性、非主要组织相容性复合体限制的方式对植物血凝素处理的淋巴母细胞或K562靶细胞表达其溶细胞程序。凝集素触发的细胞毒性在几天内即可实现，同时细胞分裂有限。我们的结果表明，来自BMT受者的循环CD8⁺/Leu-7⁺T细胞是体内已致敏的CTL，等待细胞激活。
