液相色谱法、毛细管电泳法和质谱法用于测定黑加仑中木葡聚糖结构的比较。
A comparison of liquid chromatography, capillary electrophoresis, and mass spectrometry methods to determine xyloglucan structures in black currants.
作者信息
Hilz Hauke, de Jong Laura E, Kabel Mirjam A, Schols Henk A, Voragen Alphons G J
机构信息
Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
出版信息
J Chromatogr A. 2006 Nov 10;1133(1-2):275-86. doi: 10.1016/j.chroma.2006.08.024. Epub 2006 Sep 7.
Different separation (HPAEC, RP-HPLC, CE) and identification (MALDI-TOF-MS, ESI-MS(n)) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC-MALDI-TOF-MS) or online (CE-ESI-MS(n), RP-HPLC-ESI-MS(n)) connection provided complementary information on xyloglucan structure. Online CE-MS and RP-HPLC-MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.
比较了不同的分离（高效阴离子交换色谱法、反相高效液相色谱法、毛细管电泳法）和鉴定（基质辅助激光解吸电离飞行时间质谱法、电喷雾质谱法）技术，以分析木葡聚糖与内切葡聚糖酶孵育后得到的寡糖。每种技术都能够分析木葡聚糖寡糖。包括离线（高效阴离子交换色谱法-基质辅助激光解吸电离飞行时间质谱法）或在线（毛细管电泳-电喷雾质谱法、反相高效液相色谱法-电喷雾质谱法）联用在内的几种技术提供了有关木葡聚糖结构的互补信息。在线毛细管电泳-质谱法和反相高效液相色谱-质谱法在木葡聚糖分析中首次被描述。讨论了这些技术用于不同目的（如寡糖的结构表征或寡糖谱分析）的优缺点。黑加仑木葡聚糖具有相当简单的XXXG型结构，带有含半乳糖和岩藻糖的侧链。
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