Viberg Peter, Wahlund Karl-Gustav, Skog Kerstin
Division of Applied Nutrition and Food Chemistry, Department of Food Technology, Engineering, and Nutrition, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
J Chromatogr A. 2006 Nov 10;1133(1-2):347-52. doi: 10.1016/j.chroma.2006.08.034. Epub 2006 Sep 8.
A high through-put miniaturised separation-quantification method for the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine was developed. The limit of detection was of 0.65 fmol (0.14 pg) injected or 65 pM. Heterocyclic aromatic amines are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations and the high complexity of the matrix in which they exist (food, blood, and urine), and the often small sample volumes (capillary blood; urine, blood and milk from small animals), very sensitive and selective analytical methods are required for their detection. Miniaturization was accomplished by micro solid-phase extraction in capillaries with blue chitin as solid-phase, coupled on-line (in-capillary) to capillary electrophoresis with nanospray tandem mass spectrometric detection. This new technique provided a total analysis time of only 15 min, including extraction and separation, together with low sample and solvent consumption. Blue chitin showed high tolerance towards the urine matrix. Urine collected 12h after consumption of fried chicken contained 1.8 nmol L(-1) (0.40 pg microL(-1)) of PhIP.
开发了一种用于尿液中杂环芳香胺2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）的高通量小型化分离定量方法。检测限为进样0.65 fmol（0.14 pg）或65 pM。杂环芳香胺是烹饪过程中在富含蛋白质的食物中低水平形成的诱变和致癌化合物。由于它们存在的基质（食物、血液和尿液）浓度低且复杂性高，以及通常样本量小（毛细血管血；小动物的尿液、血液和乳汁），因此需要非常灵敏和选择性的分析方法来检测它们。通过以蓝色甲壳质为固相的毛细管微固相萃取实现小型化，并与纳米喷雾串联质谱检测的毛细管电泳在线（毛细管内）联用。这项新技术仅需15分钟的总分析时间，包括萃取和分离，同时样品和溶剂消耗低。蓝色甲壳质对尿液基质显示出高耐受性。食用炸鸡12小时后收集的尿液中含有1.8 nmol L(-1)（0.40 pg μL(-1)）的PhIP。
