Iwanami Kentaro, Mita Hajime, Yamamoto Yasuhiko, Fujise Yoshihiro, Yamada Tadasu, Suzuki Tomohiko
Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 2006 Oct;145(2):249-56. doi: 10.1016/j.cbpb.2006.08.002. Epub 2006 Aug 10.
We determined the myoglobin (Mb) cDNA sequences of nine cetaceans, of which six are the first reports of Mb sequences: sei whale (Balaenoptera borealis), Bryde's whale (Balaenoptera edeni), pygmy sperm whale (Kogia breviceps), Stejneger's beaked whale (Mesoplodon stejnegeri), Longman's beaked whale (Indopacetus pacificus), and melon-headed whale (Peponocephala electra), and three confirm the previously determined chemical amino acid sequences: sperm whale (Physeter macrocephalus), common minke whale (Balaenoptera acutorostrata) and pantropical spotted dolphin (Stenella attenuata). We found two types of Mb in the skeletal muscle of pantropical spotted dolphin: Mb I with the same amino acid sequence as that deposited in the protein database, and Mb II, which differs at two amino acid residues compared with Mb I. Using an alignment of the amino acid or cDNA sequences of cetacean Mb, we constructed a phylogenetic tree by the NJ method. Clustering of cetacean Mb amino acid and cDNA sequences essentially follows the classical taxonomy of cetaceans, suggesting that Mb sequence data is valid for classification of cetaceans at least to the family level.
我们测定了9种鲸类动物的肌红蛋白（Mb）cDNA序列，其中6种是Mb序列的首次报道：塞鲸（Balaenoptera borealis）、布氏鲸（Balaenoptera edeni）、侏虎鲸（Kogia breviceps）、史氏中喙鲸（Mesoplodon stejnegeri）、朗氏中喙鲸（Indopacetus pacificus）和瓜头鲸（Peponocephala electra），另外3种则证实了先前测定的化学氨基酸序列：抹香鲸（Physeter macrocephalus）、小须鲸（Balaenoptera acutorostrata）和热带点斑原海豚（Stenella attenuata）。我们在热带点斑原海豚的骨骼肌中发现了两种类型的Mb：Mb I与蛋白质数据库中所存序列的氨基酸序列相同，而Mb II与Mb I相比，在两个氨基酸残基处存在差异。利用鲸类动物Mb的氨基酸或cDNA序列比对，我们通过邻接法构建了系统发育树。鲸类动物Mb氨基酸和cDNA序列的聚类基本遵循鲸类动物的经典分类法，这表明Mb序列数据至少在科级水平上对鲸类动物的分类是有效的。
