对自由活动大鼠的尾状核进行高频电刺激会诱导局部γ-氨基丁酸外流。
Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats.
作者信息
Hiller A, Loeffler S, Haupt C, Litza M, Hofmann U, Moser A
机构信息
Neurochemical Research Group, Department of Neurology, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
出版信息
J Neurosci Methods. 2007 Jan 30;159(2):286-90. doi: 10.1016/j.jneumeth.2006.07.023. Epub 2006 Sep 11.
Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the gamma-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats.
对内侧苍白球或丘脑底核进行高频电刺激对晚期帕金森病具有有益的运动效果。然而，这些临床结果背后的机制仍不清楚。从先前的体外研究推测，γ-氨基丁酸（GABA）系统参与了高频电刺激（HFS）的有效性。在这些实验中，我们建立了一个体内模型，该模型允许在自由活动大鼠的尾状核中通过124Hz的电脉冲同时进行并置的微透析和高频电刺激。采用微透析技术采集GABA和谷氨酸流出液，并在柱前邻苯二甲醛亚硫酸盐衍生化后，使用高效液相色谱电化学检测法进行定量。最为显著的结果是，我们能够证明高频刺激可显著增加自由活动大鼠的基础GABA流出量，而不影响谷氨酸水平。
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