Zeng Bing, Zhang Xin-Quan, Fan Yan, Lan Ying, Ma Xiao, Peng Yan, Liu Wei
Department of Grassland, Sichuan Agricultural University, Ya'an, China.
Yi Chuan. 2006 Sep;28(9):1093-100. doi: 10.1360/yc-006-1093.
Inter-simple Sequence Repeat (ISSR) molecular markers were used to detect the genetic diversity among 50 materials of Dactylis glomerata collected from China and other countries. Twelve primers produced 101 polymorphic bands, averaged 8.41 bands each primer pair. The average percentage of polymorpgic bands was 86.3.8%, and the range of GS (define) was 0.6116-0.9290, indicating a rich genetic diversity of D. glomerata. Based on the cluster and principal component analyses on the genetic characteristics, D. glomerata could be divided into 5 groups according to the nearest phylogenetic relationship. In most cases, accessions from the same continent were classified into the same group, the accessions from China and the United States belong to the different groups, respectively, indicating the geographical distribution of genetic diversity of D. glomerata. The present paper also discussed collection and conservation of germplasm resources in D. glomerata.
利用简单序列重复区间（ISSR）分子标记检测了来自中国和其他国家的50份鸭茅材料的遗传多样性。12条引物共产生101条多态性条带，平均每条引物对产生8.41条带。多态性条带的平均百分比为86.38%，遗传相似系数（GS）范围为0.6116 - 0.9290，表明鸭茅具有丰富的遗传多样性。基于遗传特征的聚类分析和主成分分析，鸭茅可根据最近的系统发育关系分为5组。在大多数情况下，来自同一大陆的材料被归为同一组，来自中国和美国的材料分别属于不同的组，这表明了鸭茅遗传多样性的地理分布。本文还讨论了鸭茅种质资源的收集和保存。
