Madesis P, Abraham E M, Kalivas A, Ganopoulos Iota, Tsaftaris A
Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
Laboratory of Range Science, Department of Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece
Genet Mol Res. 2014 Jun 9;13(2):4226-40. doi: 10.4238/2014.June.9.8.
Dactylis glomerata L. is an important forage species in the Mediterranean region, and in other regions with a similar climate. Genetic material from 3 locations in north, central, and south Greece was studied, using morphological traits, SSR, and ISSR molecular markers. Morphological analysis revealed differences among the geographic locations studied for all morphological traits, except the number of reproductive tillers. Moreover, the highest phenotypic variation was observed on the accessions from south, while the lowest was observed on the accessions from the north. Although the results of the molecular marker analysis are indicative, a high level of genetic diversity at the species level was revealed by ISSRs (GST=0.291) and SSRs (FST=0.186). Analysis of molecular variance showed that a high level of genetic diversity existed for ISSRs and SSRs within populations (62 and 83%, respectively), rather than among populations (38 and 17%, respectively). Cluster analysis divided the 3 populations in 2 groups, with the population originating from the island of Crete forming 1 group, while the populations from north Greece (Taxiarchis) and central Greece (Pertouli) were clustered in a 2nd group. In general, the results indicate that SSRs are more informative compared to ISSRs about the genetic variation within a population, whereas the ISSRs were more informative about the genetic diversity among populations However, a similar trend in diversity (genotypic and phenotypic) was observed in the morphological traits and microsatellite-based (SSR/ISSR) markers at the locations studied.
鸭茅是地中海地区以及其他气候相似地区的一种重要饲草物种。利用形态性状、SSR和ISSR分子标记，对希腊北部、中部和南部3个地点的遗传材料进行了研究。形态分析表明，除生殖分蘖数外，所研究的所有形态性状在不同地理位置之间均存在差异。此外，在来自南部的材料中观察到最高的表型变异，而在来自北部的材料中观察到最低的表型变异。虽然分子标记分析结果具有指示性，但ISSR（GST=0.291）和SSR（FST=0.186）揭示了该物种水平上的高度遗传多样性。分子方差分析表明，ISSR和SSR在种群内存在高度遗传多样性（分别为62%和83%），而非在种群间（分别为38%和17%）。聚类分析将3个种群分为2组，来自克里特岛的种群形成一组，而来自希腊北部（塔西亚基斯）和希腊中部（佩尔图利）的种群聚类为第二组。总体而言，结果表明，与ISSR相比，SSR在种群内遗传变异方面提供的信息更多，而ISSR在种群间遗传多样性方面提供的信息更多。然而，在所研究的地点，形态性状和基于微卫星的（SSR/ISSR）标记在多样性（基因型和表型）方面观察到类似趋势。
