Alejandro R, Strasser S, Zucker P F, Mintz D H
University of Miami School of Medicine, Diabetes Research Institute, Florida 33136.
Transplantation. 1990 Aug;50(2):207-10. doi: 10.1097/00007890-199008000-00007.
We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 microliters of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following autotransplantation.
我们描述了一种用于分离大量高纯度犬胰腺胰岛的方法。该方法利用酶和机械手段破坏胰腺，随后在牛血清白蛋白的不连续密度梯度上进行胰岛纯化。通过使用Cobe 2991细胞处理器，纯化过程已实现半自动化。胰岛制剂的纯度为86.5%，含有36.8微升内分泌组织。通过该方法分离的胰岛功能完好，自体移植后可恢复空腹血糖正常水平。
