Bera Rabindranath, Nayak Abhijit, Sarkar Chinmoy, Singh Suman Kumar, Ratha Jagnyeswar, Bhadra Ranjan
Department of Cellular Biochemistry, Indian Institute of Chemical Biology, Kolkata, India.
FEMS Immunol Med Microbiol. 2006 Oct;48(1):107-15. doi: 10.1111/j.1574-695X.2006.00131.x.
A novel nontoxic lipopolysaccharide (LPS) was purified from Acidiphilium strain GS18h/ATCC55963. The chemical composition of the lipid A part of this LPS is distinctly different from that of known lipid A molecules. The LPS was investigated to determine its capacity to provide protection against toxic LPS or endotoxic shock, as has been reported for other nontoxic LPSs (Rhodobacter sphaeroides and Rhodobacter capsulatus), and also the extent and type of immunomodulatory response in terms of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-beta), and IL-6 release as well as NO secretion by stimulated monocyte-macrophage systems. This study demonstrates clearly that mice immunized or primed with this LPS are fully protected against challenge with toxic Escherichia coli LPS. Unlike most of the extensively studied nontoxic LPSs, this LPS induced reactive nitrogen intermediates and released TNF-alpha, IL-beta and IL-6 in both mouse and human monocyte-macrophage systems. However, the extent of the cytokine and lymphokine releasing response was well below the range of the toxic LPS, for example that of E. coli. Owing to its capacity to provide immunostimulation of the host without causing any lethality to ensure protection against endotoxic shock, this LPS appears to have potential therapeutic value.
从嗜酸菌菌株GS18h/ATCC55963中纯化出一种新型无毒脂多糖（LPS）。该LPS脂质A部分的化学组成与已知脂质A分子明显不同。对该LPS进行了研究，以确定其提供针对有毒LPS或内毒素休克的保护能力，正如其他无毒LPS（球形红杆菌和荚膜红杆菌）所报道的那样，同时还研究了在肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-β）和IL-6释放以及刺激的单核细胞-巨噬细胞系统分泌一氧化氮方面的免疫调节反应的程度和类型。这项研究清楚地表明，用这种LPS免疫或致敏的小鼠能完全抵御有毒大肠杆菌LPS的攻击。与大多数经过广泛研究的无毒LPS不同，这种LPS在小鼠和人类单核细胞-巨噬细胞系统中均诱导产生反应性氮中间体并释放TNF-α、IL-β和IL-6。然而，细胞因子和淋巴因子释放反应的程度远低于有毒LPS（如大肠杆菌LPS）的范围。由于其能够在不造成任何致死性的情况下对宿主进行免疫刺激以确保抵御内毒素休克，这种LPS似乎具有潜在的治疗价值。
