对10个携带北极狐和貉基因组中基因及微卫星的犬BAC克隆进行荧光原位杂交图谱分析。

FISH mapping of 10 canine BAC clones harbouring genes and microsatellites in the arctic fox and the Chinese raccoon dog genomes.

作者信息

Szczerbal I, Klukowska-Roetzler J, Dolf G, Schelling C, Switonski M

机构信息

Department of Animal Genetics and Breeding, August Cieszkowski Agricultural University of Poznan, Poznan, Poland.

出版信息

J Anim Breed Genet. 2006 Oct;123(5):337-42. doi: 10.1111/j.1439-0388.2006.00608.x.

DOI:10.1111/j.1439-0388.2006.00608.x

PMID:16965407

Abstract

Cytogenetic mapping of the arctic fox and the Chinese raccoon dog were performed using a set of canine probes derived from the Bacterial Artificial Chromosome (BAC) library. Altogether, 10 BAC clones containing sequences of selected genes (PAX3, HBB, ATP2A2, TECTA, PIT1, ABCA4, ESR2, TPH1, HTR2A, MAOA) and microsatellites were mapped by fluorescence in situ hybridization (FISH) experiments to chromosomes of the canids studied. At present, the cytogenetic map on the arctic fox and Chinese raccoon dog consists of 45 loci each. Chromosomal localization of the BAC clones was in agreement with data obtained by earlier independent comparative chromosome painting. However, two events of telomere-to-centromere inversions were tentatively identified while compared with assignments in the dog karyotype.

摘要

利用一组源自细菌人工染色体（BAC）文库的犬类探针，对北极狐和貉进行了细胞遗传学图谱绘制。总共通过荧光原位杂交（FISH）实验，将10个包含选定基因（PAX3、HBB、ATP2A2、TECTA、PIT1、ABCA4、ESR2、TPH1、HTR2A、MAOA）序列的BAC克隆和微卫星定位到所研究犬科动物的染色体上。目前，北极狐和貉的细胞遗传学图谱各包含45个位点。BAC克隆的染色体定位与早期独立比较染色体涂染获得的数据一致。然而，与犬核型中的定位相比，初步确定了两例端粒到着丝粒的倒位事件。