Ihara Y, Kondo J, Miura R, Nakagawa Y, Mori H, Honda T
Second Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Gerontology. 1990;36 Suppl 1:15-24. doi: 10.1159/000213229.
The two antisera to paired helical filaments (PHF) and tau, which stained neurofibrillary tangles similarly in tissue section, were characterized. Anti-PHF reacted exclusively with the carboxyl terminal portion of tau, whereas antitau reacted with the amino terminal portion. In the blots of PHF prepared in Sarkosyl (Sarkosyl-PHF), anti-PHF stained an extensive smear from the top to the bottom of the gel, while antitau did not stain this kind of smear but labeled a small amount of intact tau that was bound to Sarkosyl-PHF. An LDS- or SDS-insoluble residue of Sarkosyl-PHF was labeled with anti-PHF, but not with antitau. Antitau-defined epitopes were susceptible to protease, whereas anti-PHF-defined epitopes were unaffected. Based on these observations, we conclude that Sarkosyl-PHF were composed largely of the carboxyl terminal region of tau and accompanied by a small amount of intact tau on their surfaces.
对两种与成对螺旋丝（PHF）和tau蛋白产生反应的抗血清进行了特性分析，这两种抗血清在组织切片中对神经原纤维缠结的染色情况相似。抗PHF血清仅与tau蛋白的羧基末端部分发生反应，而抗tau血清则与氨基末端部分发生反应。在十二烷基肌氨酸钠（Sarkosyl）制备的PHF（Sarkosyl-PHF）印迹中，抗PHF血清在凝胶的顶部到底部染出一条广泛的条带，而抗tau血清未染出这种条带，但标记了少量与Sarkosyl-PHF结合的完整tau蛋白。Sarkosyl-PHF的十二烷基二甲基溴化铵（LDS）或十二烷基硫酸钠（SDS）不溶性残留物用抗PHF血清标记，但不用抗tau血清标记。抗tau血清确定的表位易受蛋白酶影响，而抗PHF血清确定的表位则不受影响。基于这些观察结果，我们得出结论，Sarkosyl-PHF主要由tau蛋白的羧基末端区域组成，其表面伴有少量完整的tau蛋白。
