Gao Jian-Feng, Li Jian-Sheng, Zhou You-Long, Liu Ke
Institute of Geratology, Henan College of Traditional Chinese Medicine, Zhengzhou, Henan Province 430061, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Sep;4(5):530-4. doi: 10.3736/jcim20060519.
To study the effects of Naomaitong Recipe, a compound Chinese herbal medicine, on the expressions of nuclear factor-kappaB (NF-kappaB) and nitric-oxide synthases (NOSs) in brain tissue after focal cerebral ischemia reperfusion in aged rats and the dosage-effect relationship.
We adopted the middle cerebral artery occlusion (MCAO) in aged rats as the focal cerebral ischemia model, with 3-hour ischemia and 12-day reperfusion, and observed the changes of the nerve dysfunction score, the water content of cerebral constitution and the expressions of NF-kappaB, heat-shock protein 70 (HSP70) and NOSs in cerebral cortex.
The nerve dysfunction score, the water content of cerebral constitution, the expressions of NF-kappaB, HSP70, neuronal nitric-oxide synthase (nNOS), endothelial nitric-oxide synthase (eNOS) and inducible nitric-oxide synthase (iNOS) in the untreated group were higher than those of the sham-operated group. The nerve dysfunction score, the water content of cerebral constitution, the expressions of NF-kappaB, iNOS and nNOS were lower, while the expressions of HSP70 and eNOS were higher in the high-, medium- and low-dose Naomaitong-treated groups and nimodipine-treated group than those of the untreated-group. The nerve dysfunction score and the expression of nNOS were lower, while the expression of eNOS was higher in the medium-dose Naomaitong-treated group than those of the nimodipine-treated group. The expressions of HSP70 and eNOS were higher, while the other indices were lower in the medium-dose Naomaitong-treated group than those of the low-dose Naomaitong-treated group.
Naomaitong Recipe can protect the brain cells from damage after focal cerebral ischemia reperfusion injury by adjusting the expressions of NF-kappaB, HSP70 and NOSs, and the medium-dose Naomaitong Recipe is more effective.
研究中药复方脑脉通方对老年大鼠局灶性脑缺血再灌注后脑组织中核因子-κB（NF-κB）和一氧化氮合酶（NOSs）表达的影响及其量效关系。
采用老年大鼠大脑中动脉闭塞（MCAO）法制备局灶性脑缺血模型，缺血3小时，再灌注12天，观察神经功能缺损评分、脑组织含水量及大脑皮质中NF-κB、热休克蛋白70（HSP70）和NOSs的表达变化。
未治疗组神经功能缺损评分、脑组织含水量、NF-κB、HSP70、神经元型一氧化氮合酶（nNOS）、内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）的表达均高于假手术组。高、中、低剂量脑脉通治疗组和尼莫地平治疗组的神经功能缺损评分、脑组织含水量、NF-κB、iNOS和nNOS的表达均低于未治疗组，而HSP70和eNOS的表达高于未治疗组。中剂量脑脉通治疗组的神经功能缺损评分和nNOS的表达低于尼莫地平治疗组，而eNOS的表达高于尼莫地平治疗组。中剂量脑脉通治疗组的HSP70和eNOS的表达高于低剂量脑脉通治疗组，而其他指标低于低剂量脑脉通治疗组。
脑脉通方可通过调节NF-κB、HSP70和NOSs的表达，保护局灶性脑缺血再灌注损伤后的脑细胞，且中剂量脑脉通方效果更佳。
