Powell Randy L, Reyes Steven R, Lannutti Dominic I
Department of Biology, Texas A&M University-Kingsville, Kingsville, TX 78363, USA.
Toxicon. 2006 Dec 15;48(8):1095-7. doi: 10.1016/j.toxicon.2006.07.035. Epub 2006 Aug 10.
The problem of species identification in toxinological research and solutions such as molecular barcoding and DNA extraction from venom samples are addressed. Molecular barcoding is controversial with both perceived advantages and inherent problems. A method of species identification utilizing mitochondrial DNA from venom has been identified. This method could result in deemphasizing the importance of obtaining detailed information on the venom source prior to analysis. Additional concerns include; a cost prohibitive factor, intraspecific venom variation, and venom processing issues. As researchers demand more stringent records and verification, venom suppliers may be prompted to implement improved methods and controls.
本文探讨了毒理学研究中的物种鉴定问题以及诸如分子条形码和从毒液样本中提取DNA等解决方案。分子条形码存在争议,既有明显的优势,也有内在的问题。一种利用毒液中的线粒体DNA进行物种鉴定的方法已被确定。这种方法可能会导致在分析之前淡化获取毒液来源详细信息的重要性。其他问题包括成本过高、种内毒液变异以及毒液处理问题。随着研究人员要求更严格的记录和验证,毒液供应商可能会被促使采用改进的方法和控制措施。