Sasaki Hiraku, Nonaka Jun, Sasaki Takako, Nakai Yutaka
Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko, Miyagi, 989-6711, Japan.
J Ind Microbiol Biotechnol. 2007 Feb;34(2):105-10. doi: 10.1007/s10295-006-0172-6. Epub 2006 Sep 12.
We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4',6'-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.
我们从家畜粪便处理系统中分离出氨同化微生物，并评估了它们的氨同化能力。当许多分离菌株在添加了灭菌泻湖提取物的氮限制培养基中纯培养时，它们能高速利用氨。一些固定在聚乙烯醇（PVA）中的分离菌株能够利用含有活性泻湖微生物的培养基中的氨。用4',6'-二脒基-2-苯基吲哚（DAPI）染色表明，固定化的高氨同化分离菌株在PVA珠粒中占主导生长。通过对16S核糖体DNA进行部分测序，在含有活性泻湖微生物的混合培养物中，高氨同化分离菌株被鉴定为假单胞菌属和根瘤菌科的成员。
