Karp Ethan S, Inbaraj Johnson J, Laryukhin Mikhail, Lorigan Gary A
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA.
J Am Chem Soc. 2006 Sep 20;128(37):12070-1. doi: 10.1021/ja064077k.
This communication reports for the first time the determination of the helical tilt of an integral membrane peptide inserted into aligned phospholipids bilayer nanotube arrays using spin label EPR spectroscopy. Also, we demonstrate herein how the helical tilt of the peptide can be easily calculated using the hyperfine splitting values gleaned from a perpendicularly aligned bilayer in phospholipid bilayer nanotube arrays. EPR spectral simulations were used to verify the method.
本通讯首次报道了使用自旋标记电子顺磁共振光谱法测定插入排列好的磷脂双层纳米管阵列中的整合膜肽的螺旋倾斜度。此外，我们在此展示了如何利用从磷脂双层纳米管阵列中垂直排列的双层膜获取的超精细分裂值轻松计算肽的螺旋倾斜度。电子顺磁共振光谱模拟用于验证该方法。
