微流控装置中脂质双分子层膜的自动形成。

Automated formation of lipid-bilayer membranes in a microfluidic device.

作者信息

Malmstadt Noah, Nash Michael A, Purnell Robert F, Schmidt Jacob J

机构信息

Department of Bioengineering, Cybernetics Interdepartmental Program, and California Nanosystems Institute, University of California Los Angeles, Los Angeles, California 90095, USA.

出版信息

Nano Lett. 2006 Sep;6(9):1961-5. doi: 10.1021/nl0611034.

DOI:10.1021/nl0611034

PMID:16968008

Abstract

Although membrane channel proteins are important to drug discovery and hold great promise as engineered nanopore sensing elements, their widespread application to these areas has been limited by difficulties in fabricating planar lipid-bilayer membranes. We present a method for forming these sub-5-nm-thick free-standing structures based on a self-assembly process driven by solvent extraction in a microfluidic channel. This facile automatable process forms high-quality membranes able to host channel proteins measurable at single-molecule conductance resolution.

摘要

尽管膜通道蛋白对药物发现很重要，并且作为工程化纳米孔传感元件具有巨大潜力，但它们在这些领域的广泛应用受到制造平面脂质双层膜困难的限制。我们提出了一种基于微流控通道中溶剂萃取驱动的自组装过程来形成这些厚度小于5纳米的独立结构的方法。这个简便的自动化过程能够形成高质量的膜，能够容纳以单分子电导分辨率进行测量的通道蛋白。