Selwyn M J, Ng C L, Choo H L
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
FEBS Lett. 1990 Aug 20;269(1):205-8. doi: 10.1016/0014-5793(90)81155-h.
Stimulation of the rat liver mitochondrial inner-membrane anion-conducting channel by aeration is dependent on the concentration of Ca2+ ions in the assay medium. Ca2+ activates anion conduction in both aerated and non-energised mitochondria but acts over a wider concentration range and produces a greater increase in anion-conductivity in aerated mitochondria. EGTA reverses Ca2+ stimulation but takes several seconds to act, indicating slow release of Ca2+ from the activation site possibly on the matrix side of the inner mitochondrial membrane. It is suggested that this channel may respond to hormone-induced changes in cytosolic Ca2+ concentration.
通气对大鼠肝线粒体内膜阴离子传导通道的刺激作用取决于测定介质中Ca2+离子的浓度。Ca2+可激活通气的和未供能的线粒体中的阴离子传导，但作用的浓度范围更广，且在通气的线粒体中使阴离子电导率增加得更多。乙二醇双（2-氨基乙基醚）四乙酸（EGTA）可逆转Ca2+的刺激作用，但需数秒才起作用，这表明Ca2+可能从线粒体内膜基质侧的激活位点缓慢释放。有人提出，该通道可能对激素诱导的胞质Ca2+浓度变化作出反应。
