Bot F J, van Eijk L, Schipper P, Backx B, Löwenberg B
Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Leukemia. 1990 May;4(5):325-8.
The human multilineage hematopoietic growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF) induces multipotent, erythroid, and eosinophil colony formation from highly enriched normal bone marrow cells. We have examined the effects of GM-CSF combined with granulocyte-CSF (G-CSF) or macrophage-CSF (M-CSF) on the monolineage granulocytic, eosinophilic, and macrophage progenitor cells (CFU-G, CFU-Eo, and CFU-M) in accessory cell depleted marrow fractions. GM-CSF effects were assessed in direct comparison with those of interleukin-3 (IL-3) plus G-CSF or M-CSF. GM-CSF strongly synergized with G-CSF in the formation of granulocytic colonies with respect to number and size and enhanced the in vitro survival of CFU-G. More immature cells were present in colonies induced by the mixture of GM-CSF and G-CSF than by G-CSF alone. GM-CSF also synergized with M-CSF in the formation of macrophage colonies (number and size). The addition of G-CSF and M-CSF did not influence eosinophil colony formation induced by GM-CSF or IL-3. Experiments directly comparing GM-CSF and IL-3 revealed that the effects of GM-CSF on G and M colony-forming cells were significantly greater than those of IL-3. The potent positive effects between GM-CSF and G-CSF as well as between GM-CSF and M-CSF provide a powerful mechanism of amplification of granulopoiesis and monocytopoiesis.
人类多谱系造血生长因子粒细胞-巨噬细胞集落刺激因子（GM-CSF）可诱导高度富集的正常骨髓细胞形成多能、红系和嗜酸性粒细胞集落。我们研究了GM-CSF与粒细胞集落刺激因子（G-CSF）或巨噬细胞集落刺激因子（M-CSF）联合使用对去除辅助细胞的骨髓组分中单核系粒细胞、嗜酸性粒细胞和巨噬细胞祖细胞（CFU-G、CFU-Eo和CFU-M）的影响。将GM-CSF的作用与白细胞介素-3（IL-3）加G-CSF或M-CSF的作用进行直接比较来评估GM-CSF的作用。GM-CSF与G-CSF在粒细胞集落形成的数量和大小方面有强烈协同作用，并提高了CFU-G的体外存活率。与单独使用G-CSF相比，GM-CSF和G-CSF混合物诱导形成的集落中存在更多未成熟细胞。GM-CSF与M-CSF在巨噬细胞集落形成（数量和大小）方面也有协同作用。添加G-CSF和M-CSF不影响GM-CSF或IL-3诱导的嗜酸性粒细胞集落形成。直接比较GM-CSF和IL-3的实验表明，GM-CSF对粒细胞和巨噬细胞集落形成细胞的作用明显大于IL-3。GM-CSF与G-CSF以及GM-CSF与M-CSF之间的强效正性作用为粒细胞生成和单核细胞生成的放大提供了强大机制。
