Tagawa S, Doi S, Taniwaki M, Abe T, Kanayama Y, Nojima J, Matsubara K, Kitani T
Department of Clinical Research, Osaka University, Japan.
Leukemia. 1990 Aug;4(8):600-5.
We established two human plasma cell lines, FR4 and AD3, from ascitic fluid in a patient with IgA k plasmacytoma (PC). Aberrant amylase production was found in this patient. Both AD3 and FR4 were free of Epstein-Barr virus, and both produced Ig A k in vitro. They produced amylase of the salivary type in vitro. This was confirmed by the demonstration of amylase mRNA comigrating with salivary gland mRNA. These cell lines commonly had unusual chromosomal abnormalities der(14)t(8;14) and dic(8)t(1;8). AD3 had additional chromosomal abnormalities compared with FR4. This suggests that AD3 is a subline of FR4. The oncogene c-myc is rearranged in most case of Burkitt's lymphoma with t(8;14). However, neither rearrangement nor amplification of the c-myc allele was detected in our PC lines. These lines expressed c-myc of 2.4 kb. There were no structural changes in the amylase genes of AD3 and FR4 detectable with Southern blotting analysis. As these lines were authentic PC lines, they would be useful for the future study of the relationship between the mechanism of oncogenesis and the rare tumor aberration, amylase production.
我们从一名患有IgA κ浆细胞瘤（PC）患者的腹水中建立了两个人类浆细胞系，FR4和AD3。在该患者中发现了异常淀粉酶产生。AD3和FR4均无爱泼斯坦-巴尔病毒，且二者在体外均产生IgA κ。它们在体外产生唾液型淀粉酶。通过证明淀粉酶mRNA与唾液腺mRNA共迁移对此进行了证实。这些细胞系通常具有异常的染色体异常，即der(14)t(8;14)和dic(8)t(1;8)。与FR4相比，AD3还有其他染色体异常。这表明AD3是FR4的一个亚系。在大多数伴有t(8;14)的伯基特淋巴瘤病例中，癌基因c-myc发生重排。然而，在我们的浆细胞系中未检测到c-myc等位基因的重排或扩增。这些细胞系表达2.4 kb的c-myc。用Southern印迹分析未检测到AD3和FR4的淀粉酶基因有结构变化。由于这些细胞系是真正的浆细胞系，它们将有助于未来研究肿瘤发生机制与罕见肿瘤异常——淀粉酶产生之间的关系。
