[中国男性中DNA聚合酶γ的CAG重复序列以及特发性男性不育与CAG重复序列的关系]
[CAG repeats of DNA polymerase gamma in Chinese males and relationship of idiopathic male infertility to CAG repeats].
作者信息
Yao Nan, Zheng Jun-Feng, Peng Yi-Feng, Li Zuo-Xiang, Peng Zuo-Qi, Jin Xiao-Hua, Ma Xu
机构信息
Department of Medical Genetics, Peking Union Medical College, Beijing 100730, China.
出版信息
Zhonghua Nan Ke Xue. 2006 Aug;12(8):681-4, 688.
OBJECTIVE
To analyze the polymerase gamma(Polg) gene polymorphisms in Chinese idiopathic infertile males and evaluate the correlation of the polymorphisms with male infertility.
METHODS
We conducted a study of Polg CAG repeats in the sperm or blood DNA of 55 asthenospermia patients, 57 oligospermia patients, 34 azoospermia patients, and 104 controls with PCR and GeneScan. Phenotypic data were available in all the subjects, including semen parameters, and clinical profiles.
RESULTS
The frequency of 10/not 10 CAG genotype in asthenospermia patients was higher than in the other groups, but with no significance.
CONCLUSION
Our findings have shown for the first time that there exits an ethnic difference between Chinese and European males in the number of CAG repeats of Polg gene, and that 10/not 10 CAG genotype may affect sperm motility.
目的
分析中国特发性不育男性中聚合酶γ（Polg）基因多态性，并评估这些多态性与男性不育的相关性。
方法
我们采用聚合酶链反应（PCR）和基因扫描技术，对55例弱精子症患者、57例少精子症患者、34例无精子症患者以及104例对照者的精子或血液DNA中的Polg CAG重复序列进行了研究。所有受试者均有包括精液参数和临床资料在内的表型数据。
结果
弱精子症患者中10/非10 CAG基因型的频率高于其他组，但无统计学意义。
结论
我们的研究结果首次表明，中国男性与欧洲男性在Polg基因CAG重复序列数量上存在种族差异，并且10/非10 CAG基因型可能影响精子活力。
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