Chen Pei-Ni, Kuo Wu-Hsien, Chiang Chui-Liang, Chiou Hui-Ling, Hsieh Yih-Shou, Chu Shu-Chen
Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No. 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan.
Chem Biol Interact. 2006 Nov 7;163(3):218-29. doi: 10.1016/j.cbi.2006.08.003. Epub 2006 Aug 17.
Tumor metastasis is the most important cause of cancer death and various treatment strategies have targeted on preventing the occurrence of metastasis. Anthocyanins are natural colorants belonging to the flavonoid family, and are wildly used for their antioxidant properties. Here, we provided molecular evidence associated with the anti-metastatic effects of peonidin 3-glucoside and cyanidin 3-glucoside, major anthocyanins extracted from black rice (Oryza sativa L. indica), by showing a marked inhibition on the invasion and motility of SKHep-1 cells. This effect was associated with a reduced expression of matrix metalloproteinase (MMP)-9 and urokinase-type plasminogen activator (u-PA). Peonidin 3-glucoside and cyanidin 3-glucoside also exerted an inhibitory effect on the DNA binding activity and the nuclear translocation of AP-1. Furthermore, these compounds also exerted an inhibitory effect of cell invasion on various cancer cells (SCC-4, Huh-7, and HeLa). Finally, anthocyanins from O. sativa L. indica (OAs) were evidenced by its inhibition on the growth of SKHep-1 cells in vivo.
肿瘤转移是癌症死亡的最重要原因，各种治疗策略都旨在预防转移的发生。花青素是属于黄酮类家族的天然色素，因其抗氧化特性而被广泛使用。在此，我们通过显示对SKHep-1细胞的侵袭和运动有显著抑制作用，提供了与芍药素3-葡萄糖苷和矢车菊素3-葡萄糖苷（从黑米（Oryza sativa L. indica）中提取的主要花青素）的抗转移作用相关的分子证据。这种作用与基质金属蛋白酶（MMP）-9和尿激酶型纤溶酶原激活剂（u-PA）的表达降低有关。芍药素3-葡萄糖苷和矢车菊素3-葡萄糖苷也对AP-1的DNA结合活性和核转位发挥抑制作用。此外，这些化合物对各种癌细胞（SCC-4、Huh-7和HeLa）的细胞侵袭也有抑制作用。最后，籼稻（O. sativa L. indica）中的花青素（OAs）通过其对体内SKHep-1细胞生长的抑制作用得到证实。
