Qiu Jian-Tai, Chang Ting-Chang, Lin Cheng-Tao, Chen Yi-Ming, Li Frank Q, Soong Yung-Kuei, Lai Chyong-Huey
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, and Department of Life Science, Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
Vaccine. 2007 Jan 4;25(2):253-63. doi: 10.1016/j.vaccine.2006.07.034. Epub 2006 Aug 4.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immunomodulatory cytokine. Here we generated a novel codon-optimized murine GM-CSF gene as an adjuvant. The codon-optimized GM-CSF gene significantly increased protein expression levels in all cells tested. Although injection of the wild-type GM-CSF plasmids adjuvanted HIV-1 Gag DNA vaccine induced detectable immune responses, co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the DNA vaccine resulted in a strong antibody and CTL responses and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag. This novel codon-optimized GM-CSF gene offers a practical molecular strategy for potentiating immune responses to vaccines as well as other immunotherapeutic strategies.
粒细胞-巨噬细胞集落刺激因子（GM-CSF）是一种强效的免疫调节细胞因子。在此，我们构建了一种新型的密码子优化的小鼠GM-CSF基因作为佐剂。密码子优化的GM-CSF基因显著提高了所有测试细胞中的蛋白质表达水平。虽然注射佐剂化HIV-1 Gag DNA疫苗的野生型GM-CSF质粒可诱导可检测到的免疫反应，但将编码密码子优化的GM-CSF序列的质粒与DNA疫苗共同给药可产生强烈的抗体和CTL反应以及针对表达HIV-1 Gag的重组痘苗病毒感染的保护性免疫反应。这种新型的密码子优化的GM-CSF基因提供了一种实用的分子策略，可增强对疫苗以及其他免疫治疗策略的免疫反应。
