来曲唑诱导的大鼠多囊卵巢综合征中卵泡结构的微观特征

Microscopic characterization of follicular structures in letrozole-induced polycystic ovarian syndrome in the rat.

作者信息

Baravalle Celina, Salvetti Natalia R, Mira Gustavo A, Pezzone Natalia, Ortega Hugo H

机构信息

Department of Anatomy and Histology, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Santa Fe, Argentina.

出版信息

Arch Med Res. 2006 Oct;37(7):830-9. doi: 10.1016/j.arcmed.2006.04.006.

DOI:10.1016/j.arcmed.2006.04.006

PMID:16971221

Abstract

BACKGROUND

Our objective was to characterize the tissular distribution of relevant cytoskeletal proteins, cellular adhesion molecules and proliferation markers and conduct a histomorphometrical study of the follicular wall of letrozole-induced polycystic ovaries.

METHODS

Twenty rats were divided into two groups: a control group (C) of ten rats that received vehicle only (0.9% NaCl solution) once daily p.o. and a treatment group (T) of ten animals administered letrozole at a concentration of 1 mg/kg p.o. dissolved in 0.9% NaCl solution once daily during 21 days. Twenty four h after the last administration, all animals were sacrificed. Control animals were sacrificed in proestrous (n = 5) and diestrous (n = 5). Serum hormone levels, histomorphometrical changes and immunoexpression of intermediate filaments (vimentin, cytokeratins and desmin), cadherins and proliferation cellular nuclear antigen were examined.

RESULTS

The granulosa cell layer of cystic follicles had a greater significant immunostaining for vimentin and cytokeratins. Immunohistochemical localization of desmin was restricted to the theca externa. Positive immunoreactivity for cadherins rises gradually and significantly, together with the follicular development, and immunoreactivity was comparatively stronger in follicular cysts. A significantly higher immunostaining for PCNA cells was observed in secondary and tertiary follicles as compared with atretic and cystic follicles. An increase in the LH, FSH and testosterone serum concentrations was observed in letrozole-treated rats. Estradiol and progesterone showed a considerable reduction.

CONCLUSIONS

The changes observed are probably due to structural and functional alterations that occur during the process of cystogenesis and may be associated with important modifications in the expression of cytoskeletal proteins, cellular adhesion molecules and proliferation markers that may be essential for proper cellular functioning.

摘要

背景

我们的目的是描述相关细胞骨架蛋白、细胞黏附分子和增殖标志物的组织分布，并对来曲唑诱导的多囊卵巢卵泡壁进行组织形态计量学研究。

方法

将20只大鼠分为两组：对照组（C组）10只大鼠，每天口服一次赋形剂（0.9%氯化钠溶液）；治疗组（T组）10只动物，每天口服溶解于0.9%氯化钠溶液中的来曲唑，浓度为1mg/kg，持续21天。末次给药后24小时，处死所有动物。对照组动物在动情前期（n = 5）和动情后期（n = 5）处死。检测血清激素水平、组织形态计量学变化以及中间丝（波形蛋白、细胞角蛋白和结蛋白）、钙黏蛋白和增殖细胞核抗原的免疫表达。

结果

囊性卵泡的颗粒细胞层波形蛋白和细胞角蛋白的免疫染色显著增强。结蛋白的免疫组化定位局限于卵泡外膜。钙黏蛋白的阳性免疫反应性随着卵泡发育逐渐显著升高，在卵泡囊肿中免疫反应性相对较强。与闭锁卵泡和囊性卵泡相比，次级和三级卵泡中PCNA细胞的免疫染色显著更高。来曲唑治疗的大鼠血清LH、FSH和睾酮浓度升高。雌二醇和孕酮显著降低。