Shah Mohd Zahoor Ul Haq, Shrivastava Vinoy Kumar, Sofi Shazia, Jamous Yahya F, Khan Mohd Faiyaz, Alkholifi Faisal K, Ahmad Wasim, Mir Manzoor Ahmad
Endocrinology Unit, Department of Bioscience, Barkatullah University, Bhopal 462026, India.
Department of Bioresources, School of Biological Sciences, University of Kashmir Srinagar, Srinagar 190006, India.
Biomedicines. 2023 Mar 14;11(3):900. doi: 10.3390/biomedicines11030900.
Around the world, polycystic ovary syndrome (PCOS) is a complex endocrine-metabolic condition that typically affects 6-20% of females. Our study's major goal was to examine how chlorogenic acid (CGA) affected mice with endocrine and metabolic problems brought on by letrozole-induced PCOS. Group I served as the control for 81 days; Group II was given Letrozole (LETZ) orally at a dose of 6 mg/kg bw for 21 days to induce PCOS; Group III was given LETZ (6 mg/kg) for 21 days, followed by treatment with CGA (50 mg/kg bw daily) for 60 days. The study indicated that LETZ-treated mice displayed symptoms of PCOS, such as dyslipidemia, hyperinsulinemia, elevated testosterone, increases in inflammatory markers and malonaldehyde, and a decline in antioxidants (Ar, lhr, fshr, and esr2) in the ovaries. These alterations were affected when the mice were given CGA and were associated with reduced levels of adiponectin. Adiponectin showed interactions with hub genes, namely MLX interacting protein like (MLXIPL), peroxisome proliferator-activated receptor gamma Coactivator 1- alpha (PPARGC1), peroxisome proliferator-activated receptor gamma (Pparg), and adiponectin receptor 1 (Adipor1). Lastly, the gene ontology of adiponectin revealed that adiponectin was highly involved in biological processes. The findings from our research suggest that adiponectin has direct impacts on metabolic and endocrine facets of PCOS.
在全球范围内，多囊卵巢综合征（PCOS）是一种复杂的内分泌代谢疾病，通常影响6%-20%的女性。我们研究的主要目标是研究绿原酸（CGA）如何影响由来曲唑诱导的多囊卵巢综合征所引发的内分泌和代谢问题的小鼠。第一组作为对照组，为期81天；第二组口服来曲唑（LETZ），剂量为6 mg/kg体重，持续21天以诱导多囊卵巢综合征；第三组给予来曲唑（6 mg/kg）21天，随后用绿原酸（50 mg/kg体重/天）治疗60天。研究表明，接受来曲唑治疗的小鼠表现出多囊卵巢综合征的症状，如血脂异常、高胰岛素血症、睾酮升高、炎症标志物和丙二醛增加，以及卵巢中抗氧化剂（Ar、lhr、fshr和esr2）减少。当给小鼠服用绿原酸时，这些改变受到影响，并与脂联素水平降低有关。脂联素与关键基因相互作用，即MLX相互作用蛋白样（MLXIPL）、过氧化物酶体增殖物激活受体γ辅激活因子1-α（PPARGC1）、过氧化物酶体增殖物激活受体γ（Pparg）和脂联素受体1（Adipor1）。最后，脂联素的基因本体分析表明，脂联素高度参与生物过程。我们的研究结果表明，脂联素对多囊卵巢综合征的代谢和内分泌方面有直接影响。
