Medvedeva T E, Romanova Iu R, Gushchina M I, Rudenko L G, Gendon Iu Z, Aleksandrova G I, Klimov A I
Vopr Virusol. 1990 Mar-Apr;35(2):101-5.
Using mutants of fowl plague virus (FRV) which have single temperature-sensitive (ts) mutations in some genes, an analysis was carried out on reisolates from children of 3-6 years, vaccinated with a monovaccine from recombinant strains of influenza type A virus. The recombinants were obtained by crossing of current epidemic strains of subtypes A (HINI) and a (H3N2) with the cold-adapted (XA) ts-donor of attenuation A/Leningrad/134/47/57 (H2N2) from which they, as a rule, inherited 5 ts-mutations in genes 1 (PB2), 2 (PB1), 5 (NP), 7 (M), and 8 (NS). All the reisolates were shown to retain the ts-phenotype. However, in the recombination test some reisolates (most frequently those isolated at late periods of vaccination infection) no ts-mutations could be found in 1-3 genes coding for proteins of the polymerase complex, less frequently for NP and NS proteins but not for M proteins.
利用在某些基因中具有单个温度敏感（ts）突变的禽瘟病毒（FRV）突变体，对3至6岁儿童接种甲型流感病毒重组株单价疫苗后的再分离株进行了分析。这些重组株是通过将当前流行的A（HINI）和A（H3N2）亚型毒株与减毒的冷适应（XA）ts供体A/列宁格勒/134/47/57（H2N2）杂交获得的，通常它们从该供体继承了基因1（PB2）、2（PB1）、5（NP）、7（M）和8（NS）中的5个ts突变。所有再分离株均显示保留ts表型。然而，在重组试验中，一些再分离株（最常见的是在疫苗接种感染后期分离得到的那些）在编码聚合酶复合物蛋白的1至3个基因中未发现ts突变，较少见于NP和NS蛋白，但M蛋白中未发现。
