Schilbach K, Pollwein P, Schwab M, Handgretinger R, Treuner J, Niethammer D, Bruchelt G
Department of Hematology and Oncology, Children's Hospital, University of Tuebingen, FRG.
Biochem Biophys Res Commun. 1990 Aug 16;170(3):1242-8. doi: 10.1016/0006-291x(90)90527-t.
Expression of myc-box genes can be reduced by Interferon (c-myc in Daudi cells) or Retinoic acid (N-myc in neuroblastoma cells). Interferon did not reduce N-myc expression in neuroblastoma cells. However, after transfection of the human neuroblastoma cell line LS with a vector, providing the Cadmium inducible expression of an antisense N-myc transcript, drastic reduction of N-myc RNA was achieved in these cells by incubation with Cadmium and Interferon. Treatment with Cadmium alone resulted in a comparably small reduction of N-myc transcripts in these cells. Interferon alone did not appreciably affect N-myc expression. Reduction of N-myc was accompanied with reduced cell proliferation and morphological differentiation. It is assumed that most of the inhibitory effects observed are mediated by the Interferon inducible 2-5A system.
干扰素（如对多毛细胞白血病细胞中的c-myc）或视黄酸（如对神经母细胞瘤细胞中的N-myc）可降低myc-box基因的表达。干扰素不会降低神经母细胞瘤细胞中N-myc的表达。然而，用一个载体转染人神经母细胞瘤细胞系LS后，该载体可实现镉诱导的反义N-myc转录本表达，通过与镉和干扰素共同孵育，这些细胞中的N-myc RNA显著减少。单独用镉处理导致这些细胞中N-myc转录本的减少相对较小。单独使用干扰素对N-myc的表达没有明显影响。N-myc的减少伴随着细胞增殖的降低和形态分化。据推测，观察到的大多数抑制作用是由干扰素诱导的2-5A系统介导的。
