Quigg M, Elfvin L G, Aldskogius H
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Brain Res. 1990 Jun 4;518(1-2):173-8. doi: 10.1016/0006-8993(90)90969-i.
After injection of wheat germ agglutinin conjugated horseradish peroxidase (WGA-HRP) or choleragenoid conjugated HRP (B-HRP) into lower cervical and upper thoracic dorsal root ganglia (DRG), HRP reaction product was observed in peripheral fibers of spinal afferents and in postganglionic cell bodies of the stellate ganglion (SG) in the guinea pig. After WGA-HRP injection into C8-T3 or T5 DRG, HRP-labelled cells were observed to cluster at the rami within the SG, with peak labelling observed 36 h after injection. SG cell labelling occurred with B-HRP as well, but not with native HRP after injection into thoracic DRG. Injection of this tracer in C8 DRG gave rise to a small number of labelled cells. In contrast to the labelling pattern following thoracic or C8 DRG injections, injection of WGA-HRP or native HRP into C6 DRG, led to random SG cell labelling. We conclude that the anterograde transsynaptic transport, following injection of WGA-HRP into thoracic DRG, provides a method to selectively label a population of postganglionic sympathetic neurons within the SG. A combination of transsynaptic and retrograde transport appears to be responsible for labelling after injection into C8 DRG, whereas labelling after C6 DRG injections seems to be due primarily to retrograde transport.
将小麦胚凝集素结合辣根过氧化物酶（WGA-HRP）或霍乱类毒素结合HRP（B-HRP）注入豚鼠下颈段和上胸段背根神经节（DRG）后，在脊髓传入神经的外周纤维和星状神经节（SG）的节后细胞体中观察到HRP反应产物。将WGA-HRP注入C8-T3或T5 DRG后，观察到HRP标记的细胞聚集在SG内的分支处，注射后36小时观察到标记峰值。注射B-HRP后也出现SG细胞标记，但将天然HRP注入胸段DRG后未出现标记。将这种示踪剂注入C8 DRG会产生少量标记细胞。与胸段或C8 DRG注射后的标记模式不同，将WGA-HRP或天然HRP注入C6 DRG会导致SG细胞随机标记。我们得出结论，将WGA-HRP注入胸段DRG后的顺行跨突触运输提供了一种选择性标记SG内一群节后交感神经元的方法。注入C8 DRG后的标记似乎是由跨突触和逆行运输共同作用引起的，而注入C6 DRG后的标记似乎主要是由于逆行运输。
