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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Sams G H, Hargis B M, Hargis P S

Department of Poultry Science, Texas A & M University System, College Station 77843-2472.

Comp Biochem Physiol B. 1990;96(3):585-90. doi: 10.1016/0305-0491(90)90061-w.

Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected.

脂肪酸结合蛋白（A-FABP）从鸡脂肪细胞质溶胶中分离得到。2. 通过SDS-聚丙烯酰胺凝胶电泳测定鸡A-FABP的相对分子量为14400；其等电点为5.1；氨基酸组成数据表明与哺乳动物心脏和脂肪组织的脂肪酸结合蛋白具有结构同源性。3. 针对A-FABP制备的多克隆抗血清对鸡A-FABP表现出单特异性，未观察到与鸡肝蛋白的交叉反应。4. 相对配体结合特性的测定表明，A-FABP对亚油酸表现出最大的结合活性，其次是油酸、棕榈酰辅酶A和棕榈酸；未检测到对胆固醇的结合亲和力。

Sams G H, Hargis B M, Hargis P S

Department of Poultry Science, Texas A & M University System, College Station 77843-2472.

Comp Biochem Physiol B. 1990;96(3):585-90. doi: 10.1016/0305-0491(90)90061-w.

Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected.

脂肪酸结合蛋白（A-FABP）从鸡脂肪细胞质溶胶中分离得到。2. 通过SDS-聚丙烯酰胺凝胶电泳测定鸡A-FABP的相对分子量为14400；其等电点为5.1；氨基酸组成数据表明与哺乳动物心脏和脂肪组织的脂肪酸结合蛋白具有结构同源性。3. 针对A-FABP制备的多克隆抗血清对鸡A-FABP表现出单特异性，未观察到与鸡肝蛋白的交叉反应。4. 相对配体结合特性的测定表明，A-FABP对亚油酸表现出最大的结合活性，其次是油酸、棕榈酰辅酶A和棕榈酸；未检测到对胆固醇的结合亲和力。